FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pka-C1, ptc
FlyBase ID
FBig0000120220
Interaction Network
Interactions Browser links
Pka-C1 network
ptc network
Reported Interactions
FBrf0243776-1.coIP.WB
Reference
Fan et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

V5 or Myc tag

ptc 
ptc 
Pka-C1 

PKAc

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
intracellular domain C4
necessary binding region
ptc 
intracellular domain C4 residues 583-670
sufficient binding region
aa 583-670
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fan et al., 2019)

The ptc-Pka-C1 interaction is decreased upon co-expression of hh.

(Fan et al., 2019)
FBrf0243776-2.coIP.WB
Reference
Fan et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pka-C1 

PKAc

bait

HA tag

ptc 

Ptc

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fan et al., 2019)
FBrf0243776-4.PD.WB
Reference
Fan et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

GST tag

ptc 
Pka-C1 

PKAc

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
intracellular domain C4 residues 583-670
sufficient binding region
aa 583-670
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Fan et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)