FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Agbe, Irp-1A
FlyBase ID
FBig0000120270
Interaction Network
Interactions Browser links
Agbe network
Irp-1A network
Reported Interactions
FBrf0244135-1.coIP.WB
Reference
Huynh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Agbe 

AGBE

bait

Myc tag

Irp-1A 

IRP1A

prey

FLAG tag

Irp-1A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Irp-1A 
Fe-S cluster binding domain residue 450
mutation disrupting interaction
aa 450

C450S, coordinates relative to Irp-1A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huynh et al., 2019)
FBrf0244135-5.coIP.WB
Reference
Huynh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Irp-1A 

IRP1A

bait

FLAG tag

Agbe 

AGBE

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huynh et al., 2019)
FBrf0244135-6.coIP.MS
Reference
Huynh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / late third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Irp-1A 

IRP1A

bait

FLAG tag

Irp-1A 
Agbe 

AGBE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Irp-1A 
Fe-S cluster binding domain residue 450
mutation disrupting interaction
aa 450

C450S, coordinates relative to Irp-1A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae; bait produced from tagged knock-in construct or transgenic construct; prey produced from endogenous gene.

(Huynh et al., 2019)
FBrf0244135-9.coIP.MS
Reference
Huynh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / late third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Agbe 

AGBE

bait

FLAG tag

Irp-1A 

IRP1A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae; bait produced from tagged knock-in construct; prey produced from endogenous gene.

(Huynh et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)