FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sktl, ash2
FlyBase ID
FBig0000120306
Interaction Network
Interactions Browser links
sktl network
ash2 network
Reported Interactions
FBrf0179167-1.Y2H
Reference
Cheng and Shearn, 2004
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ash2 

ASH2

bait

fused to GAL4 DNA-binding domain

sktl 

SKTL

prey

fused to GAL4 activation domain

sktl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sktl 
interaction domain
sufficient binding region
aa 503-700
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larval cDNA expression library).

(Cheng and Shearn, 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Cheng and Shearn, 2004)
FBrf0179167-2.PD.WB
Reference
Cheng and Shearn, 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ash2 

ASH2

bait

GST tag

ash2 
sktl 

SKTL

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ash2 
interaction domain
sufficient binding region
aa 260-404
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Cheng and Shearn, 2004)
FBrf0179167-3.coIP.WB
Reference
Cheng and Shearn, 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sktl 

SKTL

bait

FLAG tag

ash2 

ASH2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Cheng and Shearn, 2004)
External Crossreferences and Linkouts ( 0 )
References (1)