FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
PIG-B, Lam
FlyBase ID
FBig0000120433
Interaction Network
Interactions Browser links
PIG-B network
Lam network
Reported Interactions
FBrf0245218-1.coIP.WB
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PIG-B 

PIG-B

bait

FLAG tag

Lam 

Lamin Dm0

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Yamamoto-Hino et al., 2020)
FBrf0245218-2.coIP.WB
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lamin Dm0

bait

HA tag

Lam 
Lam 
PIG-B 

PIG-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lam 
tail domain
sufficient binding region
aa 428-622
Lam 
tail domain
necessary binding region
aa 428-622
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Yamamoto-Hino et al., 2020)
FBrf0245218-3.coIP.MS
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PIG-B 

PIG-B

bait

FLAG tag

Lam 

Lamin Dm0

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yamamoto-Hino et al., 2020)
FBrf0245218-4.PBD
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lamin Dm0

bait
PIG-B 

PIG-B

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in CHO cell system; bait produced from transfected construct; prey produced from transfected construct.

(Yamamoto-Hino et al., 2020)

When expressed in heterologous CHO cells, Lam localizes to the nuclear envelope while PIG-B localizes to the ER. When coexpressed together, PIG-B localization changes to the nuclear envelope suggesting a Lam-PIG-B interaction.

(Yamamoto-Hino et al., 2020)
FBrf0245218-5.PL.MS
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lamin Dm0

bait

miniTurboID,V5 tag

PIG-B 

PIG-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yamamoto-Hino et al., 2020)
FBrf0245218-6.PL.WB
Reference
Yamamoto-Hino et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lamin Dm0

bait

miniTurboID,V5 tag

PIG-B 

PIG-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yamamoto-Hino et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)