FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
otk, Sema1a
FlyBase ID
FBig0000120436
Interaction Network
Interactions Browser links
otk network
Sema1a network
Reported Interactions
FBrf0245590-2.SPR
Reference
Rozbesky et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
otk 

OTK

bait
otk 
Sema1a 

Sema1a

prey
Sema1a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
otk 
Ig domains 1-5
sufficient binding region
aa 23-580

coordinates relative to otk-PA

Sema1a 
ecto domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293T cell system; prey source unspecified.

(Rozbesky et al., 2020)

Kd = 4.0uM. A K237A mutant form of otk was used to prevdnt cleavage.

(Rozbesky et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)