FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Alms1a, Klp10A
FlyBase ID
FBig0000120622
Interaction Network
Interactions Browser links
Alms1a network
Klp10A network
Reported Interactions
FBrf0246835-1.coIP.MS
Reference
Chen and Yamashita, 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp10A 

Klp10A

bait
Alms1a 

Alms1a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen and Yamashita, 2020)
FBrf0246835-2.coIP.WB
Reference
Chen and Yamashita, 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp10A 

Klp10A

bait

GFP tag

Alms1a 

Alms1a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen and Yamashita, 2020)
FBrf0246835-3.PD.WB
Reference
Chen and Yamashita, 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alms1a 

Alms1a

bait

GST tag

Alms1a 
Klp10A 

Klp10A

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Alms1a 
C-terminal domain
sufficient binding region
aa 1115-1322

coordinates relative to Alms1a-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was bacterial lysate; bait produced as a recombinant fusion protein; prey produced as a recombinant fusion protein.

(Chen and Yamashita, 2020)
FBrf0246835-4.BACTH
Reference
Chen and Yamashita, 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alms1a 

Alms1a

bait

fused to T25 fragment

Klp10A 

Klp10A

prey

fused to T18 fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was bacteria; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen and Yamashita, 2020)

Bacterial two-hybrid system: adenylate cyclase complementation.

(Chen and Yamashita, 2020)
FBrf0246835-5.BiFC.FM
Reference
Chen and Yamashita, 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alms1a 

Alms1a

bait

YFP tag, N-terminal fragment

Klp10A 

Klp10A

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Chen and Yamashita, 2020)
External Crossreferences and Linkouts ( 0 )
References (1)