FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
emb, Ubx
FlyBase ID
FBig0000120664
Interaction Network
Interactions Browser links
emb network
Ubx network
Reported Interactions
FBrf0246795-1.BiFC.FM
Reference
Duffraisse et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emb 

Emb

bait

YFP tag, N-terminal fragment

Ubx 

Ubx

prey

YFP tag, C-terminal fragment

Ubx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
NES domain
mutation decreasing interaction
aa 245,249,253

I245A,I249A,L253A coordinates relative to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Duffraisse et al., 2020)
FBrf0246795-2.PD.WB
Reference
Duffraisse et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

GST tag

Ubx 
emb 

Emb

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
NES domain
mutation decreasing interaction
aa 245,249,253

I245A,I249A,L253A coordinates relative to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Duffraisse et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)