FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Cyp40, AGO2
FlyBase ID
FBig0000120680
Interaction Network
Interactions Browser links
Cyp40 network
AGO2 network
Reported Interactions
FBrf0247227-2.coIP.WB
Reference
Iki et al., 2020
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cyp40 

Cyp40

bait

FLAG tag

Cyp40 
Cyp40 
Cyp40 
AGO2 

Ago2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cyp40 
TPR domain
sufficient binding region
aa 228-383

deletion of TPR domain, coordinates relative to Cyp40-PA

Cyp40 
TPR domain
mutation decreasing interaction
aa 175-383

coordinates relative to Cyp40-PA

Cyp40 
TPR domain residues 228,321
mutation disrupting interaction
aa 228,321

R228A,K321A, coordinates relative to Cyp40-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Iki et al., 2020)

Interaction studied in presence of ATPĪ³S to inhibit ATP hydrolysis by Hsp83.

(Iki et al., 2020)
FBrf0257100-1.PL.MS
Reference
Iki et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cyp40 

Cyp40

bait

TurboID-FLAG tag

AGO2 

Ago2

prey

Biotin tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from transgenic construct; prey produced from endogenous gene.

(Iki et al., 2023)
FBrf0257100-3.PL.PD.WB
Reference
Iki et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cyp40 

Cyp40

bait

TurboID-FLAG tag

AGO2 

Ago2

prey

Biotin tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from transgenic construct; prey produced from endogenous gene.

(Iki et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (2)