FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
2mit, Argk1
FlyBase ID
FBig0000120700
Interaction Network
Interactions Browser links
2mit network
Argk1 network
Reported Interactions
FBrf0247467-1.coIP.MS
Reference
Bozzato et al., 2020
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
2mit 

2MIT

bait

HA tag

Argk1 

ArgK

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bozzato et al., 2020)
FBrf0247467-2.Y2H
Reference
Bozzato et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
2mit 

2MIT

bait

fused to lexA DNA-binding domain

2mit 
2mit 
Argk1 

ArgK

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
2mit 
interaction domain
sufficient binding region
aa 29-930

coordinates relative to 2mit-PA

2mit 
interaction domain
necessary binding region
aa 29-930

coordinates relative to 2mit-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/B42-AD. Argk isoform PC was used in the yeast two-hybrid assay.

(Bozzato et al., 2020)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bozzato et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)