FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bol, Hsp60C
FlyBase ID
FBig0000120701
Interaction Network
Interactions Browser links
bol network
Hsp60C network
Reported Interactions
FBrf0247226-2.PD.MS
Reference
Gao et al., 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bol 

bol

bait
bol 
bol 
Hsp60C 

Hsp60C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bol 
circEx2-3
sufficient binding region
TGGATGTACTTCTAGATCCGCTTGCAGTCT
bol 
circEx4-7
sufficient binding region
GTACCACGCATTCACCCTTGGTAGTTCTGAG
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait was biotinylated antisense DNA oligo complementary to RNA of interest; prey derived from testis extract.

(Gao et al., 2020)
FBrf0247226-3.coIP.PCR
Reference
Gao et al., 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsp60C 

Hsp60C

bait

FLAG tag

bol 

bol

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gao et al., 2020)
FBrf0247226-5.coIP.PCR
Reference
Gao et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hsp60C 

Hsp60C

bait

FLAG tag

bol 

bol

prey
bol 
bol 
bol 
bol 
bol 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bol 
circEx2-3
sufficient binding region
bol 
circEx2-3 motif2
mutation decreasing interaction
CAAAAAGGAA

CAAAAAGGAA to CGAACGTGTC

bol 
circEx4-7
sufficient binding region
bol 
circEx4-7 motif1
mutation decreasing interaction
AACATTGCAC

AACATTGCAC to CGAACGTGTC

bol 
circEx4-7 motif2
mutation decreasing interaction
CAAAAAGCAG

AACATTGCAC to CGAACGTGTC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)