Double immunoprecipitation
Source was cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was tou immunoprecipitate from cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
Double immunoprecipitation - tou was immunoprecipitated from extract of cells transfected with tou, pnr and Chi. Chi was then immunoprecipitated from the proteins recovered in the first tou immunoprecipitate. The recovery of pnr and tou in the second immunoprecipitate suggests that tou, pnr and Chi form a ternary complex.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/VP16-AD. N-terminal homodimerization domain (DDT)
N-terminal homodimerization domain (DDT)
Source was cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
