Source was larvae of transgenic line; bait produced from transgenic gene; prey produced from endogenous gene.

a zip-GFP transgene was expressed in otherwise wild type larvae. Larval lysates were precipitated with anti-GFP and the resulting samples blotted with anti-zip antibodies and seperated by SDS-PAGE. zip-GFP migrated on the gel more slowly than did WT-zip.

Protein produced as a recombinant fusion protein in bacterial system.

Purified zip protein was incubated at a range of salt concentrations and centrifuged at 100,000g for 30 minutes, then soluble and insoluble phases were analzyed by SDS-PAGE and gel staining. Pelleting of zip protein was taken to indicate the formation of filaments.