FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
flfl, stwl
FlyBase ID
FBig0000120840
Interaction Network
Interactions Browser links
flfl network
stwl network
Reported Interactions
FBrf0247572-2.PD.A
Reference
Karman et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

bait

GST tag

flfl 
flfl 
stwl 

Stwl

prey

[35]S label

stwl 
stwl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
EVH1 domain
sufficient binding region
aa 1-168

coordinates relative to flfl-PD

flfl 
EVH1 domain residue 70
mutation disrupting interaction
aa 70

L70A, coordinates relative to flfl-PD

stwl 
interaction domain
sufficient binding region
aa 1-730aa 750-1037

coordinates relative to stwl-PA

coordinates relative to stwl-PA

stwl 
interaction domain residues 882,885
mutation decreasing interaction
aa 882,885

M882A,P885A, coordinates relative to Prp16-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Karman et al., 2020)
FBrf0247572-20.PD.MS
Reference
Karman et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

bait

GST tag

flfl 
stwl 

Stwl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
EVH1 domain
sufficient binding region
aa 1-168

coordinates relative to flfl-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Karman et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)