FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
arm, Src42A
FlyBase ID
FBig0000120883
Interaction Network
Interactions Browser links
arm network
Src42A network
Reported Interactions
FBrf0187486-3.coIP.WB
Reference
Takahashi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 13embryonic stage 15embryonic stage 14
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait
Src42A 

Src42A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

wild-type embryos; bait produced from endogenous gene; prey produced from endogenous gene

(Takahashi et al., 2005)

Src42A in the membrane fraction and not in the cytosolic fraction.

(Takahashi et al., 2005)
FBrf0187486-5.coIP.WB
Reference
Takahashi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 13embryonic stage 15embryonic stage 14
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait
Src42A 

Src42A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene in transgenic embryos for a dominant negative form of Src42A[Kr] or a constitutively active form Src42A[YF].; prey produced from endogenous gene.

(Takahashi et al., 2005)

Src42A interaction seen in Src42A[Kr] embryos (membrane fraction) compared to wt and Src42A[YF] embryos. Reduced interaction in cytosolic fractions for all extracts.

(Takahashi et al., 2005)
FBrf0187486-7.PD.WB
Reference
Takahashi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Src42A 

Src42A

bait

MBP tag

Src42A 
Src42A 
arm 

Arm

prey

Gst tag

arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Src42A 
interaction domain
sufficient binding region
aa 391-404

coordinates relative to Src42A-PG

Src42A 
interaction domain
necessary binding region
aa 391-404

coordinates relative to Src42A-PA

arm 
Arm repeat variant
sufficient binding region
aa 140-671

coordinates relative to arm-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Takahashi et al., 2005)

Strong GST-Arm-repeat (GST-ArmR) signals were recognized in the lanes for MBP-Src42A[Kinase] and the 14 amino acid autophosphorylation site-containing peptide.

(Takahashi et al., 2005)
External Crossreferences and Linkouts ( 0 )
References (1)