FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nelf-E, NELF-B
FlyBase ID
FBig0000120905
Interaction Network
Interactions Browser links
Nelf-E network
NELF-B network
Reported Interactions
FBrf0247767-5.coIP.WB
Reference
Mazina et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
NELF-B 

NELF B

bait
Nelf-E 

NELF E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2021)
FBrf0247767-8.coIP.WB
Reference
Mazina et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

NELF E

bait
NELF-B 

NELF B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2021)
FBrf0257712-13.PD.MW
Reference
Dollinger et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

NelfE

bait

His tag

NELF-B 

NelfB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

(Dollinger et al., 2023)

The NELF complex was tandem purified with Nelf-E affinity purification followed by immunoprecipitation with TH1-anti-FLAG antibody. Nelf-A, NELF-B, TH1, and Nelf-E captured as 3 pairwise interactions.

(Dollinger et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (2)