FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
emb, tim
FlyBase ID
FBig0000120911
Interaction Network
Interactions Browser links
emb network
tim network
Reported Interactions
FBrf0248110-1.coIP.WB
Reference
Cai et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
tim 

tim

bait

HA tag

tim 
emb 

xpo1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
phosphorylation site residue 1404
mutation increasing interaction
aa 1404

S1404A, coordinates relative to tim-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

per was coexpressed with tim and emb.

(Cai et al., 2021)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cai et al., 2021)
FBrf0248110-2.coIP.WB
Reference
Cai et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
emb 

xpo1

bait

FLAG tag

tim 

tim

prey

HA tag

tim 
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
phosphorylation site residue 1404
mutation increasing interaction
aa 1404

S1404A, coordinates relative to tim-PT

tim 
phosphorylation site residue 1404
mutation decreasing interaction
aa 1404

S1404D, coordinates relative to tim-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

per was coexpressed with tim and emb.

(Cai et al., 2021)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cai et al., 2021)
FBrf0248110-3.PD.WB
Reference
Cai et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
emb 

xpo1

bait

GST tag

tim 

tim

prey
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
phosphorylation site residue 1404
mutation increasing interaction
aa 1404

S1404A, coordinates relative to tim-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected S2 cell extract; prey derived from transgenic adult head extract.

(Cai et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)