FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
slmb, HIV-1\Vpu
FlyBase ID
FBig0000120922
Interaction Network
Interactions Browser links
slmb network
HIV-1\Vpu network
Reported Interactions
FBrf0218002-1.Y2H
Reference
Marchal et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HIV-1\Vpu 

Vpu

bait

fused to lexA DNA-binding domain

HIV-1\Vpu 
HIV-1\Vpu 
slmb 

SLIMB

prey

fused to B42 activation domain

slmb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
slmb 
WD1 domain
sufficient binding region
aa 192-242
HIV-1\Vpu 
cytoplasmic domain
sufficient binding region
aa 192-242
HIV-1\Vpu 
cytoplasmic domain
mutation disrupting interaction
aa 52,56

S52N,S56N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/B42-AD

(Marchal et al., 2012)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Marchal et al., 2012)
FBrf0218002-2.coIP.WB
Reference
Marchal et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slmb 

SLIMB

bait
HIV-1\Vpu 

Vpu

prey

HA tag

HIV-1\Vpu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
HIV-1\Vpu 
cytoplasmic domain
mutation disrupting interaction
aa 52,56

S52N,S56N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Marchal et al., 2012)
External Crossreferences and Linkouts ( 0 )
References (1)