Rept pulls down both Myc and Pont in one experiment. After Myc and Rept were pulled down by Pont-dMyc, the eluate was reprobed with anti-AU1 (Rept), which pulled down stronger bands for Myc, Pont and Rept itself.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

pont can compete with rept for Myc binding. The Myc-pont interaction is more resistant to increasing NaCl concentrations than Myc-rept.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Myc antibody pulls down Rept

Myc antibody pulls down Rept

Source was third-instar larval extracts; bait produced from endogenous gene; prey produced from endogenous gene