FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
flfl, Prp16
FlyBase ID
FBig0000120946
Interaction Network
Interactions Browser links
flfl network
Prp16 network
Reported Interactions
FBrf0247572-1.PD.A
Reference
Karman et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

bait

GST tag

flfl 
flfl 
Prp16 

Prp16

prey

[35]S label

Prp16 
Prp16 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
EVH1 domain
sufficient binding region
aa 1-168

coordinates relative to flfl-PD

flfl 
EVH1 domain residue 70
mutation disrupting interaction
aa 70

L70A, coordinates relative to flfl-PD

Prp16 
interaction domain
sufficient binding region
aa 1-134

coordinates relative to Prp16-PA

Prp16 
interaction domain residues 98,101
mutation disrupting interaction
aa 98,101

F98A,P101A, coordinates relative to Prp16-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Karman et al., 2020)
FBrf0247572-19.PD.MS
Reference
Karman et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

bait

GST tag

flfl 
Prp16 

Prp16

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
EVH1 domain
sufficient binding region
aa 1-168

coordinates relative to flfl-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Karman et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)