FB2025_05 , released December 11, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Myc, pont
FlyBase ID
FBig0000120951
Interaction Network
Interactions Browser links
Myc network
pont network
Reported Interactions
FBrf0188250-10.PD.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myc 

dMyc

bait

GST tag

Myc 
pont 

Pont

prey

cMyc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myc 
interaction domain
sufficient binding region
aa 46-507
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bellosta et al., 2005)
FBrf0188250-3.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myc 

Myc

bait

HA tag

pont 

Pont

prey

cMyc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bellosta et al., 2005)

Myc pulls down Pont

(Bellosta et al., 2005)
FBrf0188250-5.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myc 

Myc

bait
pont 

Pont

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bellosta et al., 2005)

Myc antibody pulls down Pont

(Bellosta et al., 2005)
FBrf0188250-7.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myc 

Myc

bait
pont 

Pont

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Myc antibody pulls down Pont

(Bellosta et al., 2005)

Source was third-instar larval extracts; bait produced from endogenous gene; prey produced from endogenous gene

(Bellosta et al., 2005)
FBrf0188250-8.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pont 

Pont

bait

cMyc tag recognized by antibody 9E10

Myc 

Myc

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct

(Bellosta et al., 2005)

Pont pulls down both Myc and Rept in one experiment.

(Bellosta et al., 2005)
External Crossreferences and Linkouts ( 0 )
References (1)