FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000121002
Interaction Network
Interactions Browser links
Apc2 network
CtBP network
Reported Interactions
FBrf0227580-1.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Apc2 

APC

bait

Gst-tag

Apc2 
CtBP 

dCtBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
E-APC
sufficient binding region
aa 453-1067
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

13 tryptic peptides derived from a 40 kDa protein coincided with peptides from dCtBP.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from wild-type embryonic extract.

FBrf0227580-3a.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

Gst tag

Apc2 

APC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from by in vitro translation.

FBrf0227580-4.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Apc2 

E-APC

bait

Gst tag

Apc2 
Apc2 
CtBP 

dCtBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
E-APC
sufficient binding region
aa 488-547
Apc2 
P-h-D-L-S motif
mutation disrupting interaction
aa 488-547

triple alanine substitution (AxAxA) in the P-h-D-L-S motif of each 15R (in the context of the C-terminal half of E-APC)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

External Crossreferences and Linkouts ( 0 )
References (1)