FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000121238
Interaction Network
Interactions Browser links
stnB network
Dap160 network
Reported Interactions
FBrf0187146-1.FW.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stnB 

STNB

bait

DAP-160

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EH2 domain
sufficient binding region
aa 190-276
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced as a recombinant fusion protein in bacterial system.

FBrf0187146-12.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stnB 

STNB

bait

DAP-160

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0187146-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DAP-160

bait

Gst tag

stnB 

STNB

prey

MBP tag

stnB 
stnB 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EH1 domain
sufficient binding region
aa 13-97
EH2 domain
sufficient binding region
aa 190-276
stnB 
N-terminal domain residues 1-480
sufficient binding region
aa 1-480
stnB 
C-terminal domain residues 642-1262
sufficient binding region
aa 642-1262
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The N-terminal domain of stnB binds both Dap160 EH1 and EH2 domains. The C-terminal domain of stnB binds Dap160 EH2\'s domain.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0187146-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stnB 

STNB

bait

MBP tag

stnB 

DAP-160

prey

Gst tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EH2 domain residues 190-276
sufficient binding region
aa 190-276
stnB 
N-terminal domain residues 1-480
sufficient binding region
aa 1-480
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0187146-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stnB 

STNB

bait

MBP tag

stnB 

DAP-160

prey

Gst tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SH3C domain residues 944-997
sufficient binding region
aa 944-997
EH2 domain residues 190-276
sufficient binding region
aa 190-276
stnB 
C-terminal domain residues 642-692
sufficient binding region
aa 642-692
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0187146-5.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DAP-160

bait

Gst tag

stnB 

STNB

prey

MBP tag

stnB 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SH3C domain residues 944-997
sufficient binding region
aa 944-997
stnB 
C-terminal domain residues 642-1262
sufficient binding region
aa 642-1262
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0187146-6.CS.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stnB 

STNB

neutral component
stnB 

STNB

neutral component
stnB 

STNB

neutral component

DAP-160

neutral component

DAP-160

neutral component

DAP-160

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; proteins produced from endogenous gene.

External Crossreferences and Linkouts ( 0 )
References (1)