FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Vps16A, car
FlyBase ID
FBig0000121245
Interaction Network
Interactions Browser links
Vps16A network
car network
Reported Interactions
FBrf0187837-10.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vps16A 

dVps16A

bait

HA tag

Vps16A 
car 

Car

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vps16A 
C terminal domain
sufficient binding region
aa 489-813

coordinates relative to Vps16A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pulipparacharuvil et al., 2005)

Cells were transfected with HA-tagged Vps16A-C but immunoprecipitated with anti-Vps16A antibody.

(Pulipparacharuvil et al., 2005)
FBrf0187837-11.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vps16A 

dVps16A

bait

Myc tag

Vps16A 
car 

Car

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vps16A 
C terminal domain
sufficient binding region
aa 489-813

coordinates relative to Vps16A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pulipparacharuvil et al., 2005)

Cells were transfected with Myc-tagged Vps16A-C but immunoprecipitated with anti-Vps16A antibody.

(Pulipparacharuvil et al., 2005)
FBrf0187837-12.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vps16A 

dVps16A

bait

Myc tag

Vps16A 
car 

Car

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vps16A 
Cdelta1 terminal domain
sufficient binding region
aa 1-555

coordinates relative to Vps16A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pulipparacharuvil et al., 2005)

Cells were transfected with HA tagged car but detected with anti-car antibody. Combined with previous IPs with Vps16A-C domain, the authors conclude region 489-555 is the necessary binding region of Vps16A.

(Pulipparacharuvil et al., 2005)
FBrf0187837-4.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
car 

Car

bait
Vps16A 

dVps16A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cytosolic cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Pulipparacharuvil et al., 2005)
FBrf0187837-6.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vps16A 

dVps16A

bait
car 

Car

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cytosolic cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Pulipparacharuvil et al., 2005)
FBrf0187837-7.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vps16A 

dVps16A

bait

HA tag

car 

Car

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cytosolic cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pulipparacharuvil et al., 2005)
FBrf0187837-9.coIP.WB
Reference
Pulipparacharuvil et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
car 

Car

bait

HA tag

Vps16A 

dVps16A

prey

HA tag

Vps16A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vps16A 
C terminal domain
sufficient binding region
aa 489-813

coordinates relative to Vps16A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pulipparacharuvil et al., 2005)

Cells were transfected with HA-tagged car but immunoprecipitated with anti-car antibody.

(Pulipparacharuvil et al., 2005)
External Crossreferences and Linkouts ( 0 )
References (1)