FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trp, trpl
FlyBase ID
FBig0000121264
Interaction Network
Interactions Browser links
trp network
trpl network
Reported Interactions
FBrf0097895-1.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait
trpl 

TRPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xu et al., 1997)
FBrf0097895-10.PD.A
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

GST tag

trp 
trpl 

TRPL

prey

[35]S label

trpl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Xu et al., 1997)
FBrf0097895-13.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait
trpl 

TRPL

prey

MYC tag

trpl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-15.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait

MYC tag

trpl 
trp 

TRP

prey

Flag tag

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-2.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait
trpl 

TRPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-3.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait
trp 

TRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-4.Y2H
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait

fused to GAL4 DNA-binding domain

trpl 
trp 

TRP

prey

fused to GAL4 activation domain

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Xu et al., 1997)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Xu et al., 1997)
FBrf0097895-7.Y2H
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

fused to GAL4 DNA-binding domain

trp 
trpl 

TRPL

prey

fused to GAL4 activation domain

trpl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Xu et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Xu et al., 1997)
FBrf0097895-8.PD.A
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait

GST tag

trpl 
trp 

TRP

prey

[35]S label

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
N-terminal interaction domain residues 1-338
sufficient binding region
aa 1-338

coordinates relative to trpl-PA

trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Xu et al., 1997)
External Crossreferences and Linkouts ( 0 )
References (1)