FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
PCID2, Ras64B
FlyBase ID
FBig0000121451
Interaction Network
Interactions Browser links
PCID2 network
Ras64B network
Reported Interactions
FBrf0251826-5.coIP.PCR
Reference
Glukhova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PCID2 

PCID2

bait
Ras64B 

Ras2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear and cytoplasmic cell extracts of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Glukhova et al., 2021)
FBrf0257135-1.EMSA.A
Reference
Vdovina et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PCID2 

PCID2

bait

GST tag

PCID2 
PCID2 
PCID2 
PCID2 
Ras64B 

Ras64B

prey

[32]P label

Ras64B 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ras64B 
3 prime non-coding region
sufficient binding region
nt 103-306
PCID2 
C terminal domain
mutation decreasing interaction
aa 361-395

δ 361-395; coordinates relative to PCID2-PA

PCID2 
middle domain residue 191
mutation disrupting interaction
aa 191

R191A; coordinates relative to PCID2-PA

PCID2 
middle domain residue 216
mutation disrupting interaction
aa 216

R216A; coordinates relative to PCID2-PA

PCID2 
C terminal region
sufficient binding region
aa 283-395

δ 361-395; coordinates relative to PCID2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Vdovina et al., 2023)
FBrf0258597-1.PD.WB
Reference
Vdovina et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ras64B 

ras2

bait

biotin label

PCID2 

PCID2

prey
PCID2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PCID2 
C terminal domain
mutation decreasing interaction
aa 361-395

coordinates relative to PCID2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Vdovina et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (3)