Source was membrane fraction of pupae; bait produced from endogenous gene; prey produced from endogenous gene.
Split-ubiquitin membrane yeast two-hybrid (MYTH) assay: yeast LexA-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cell system; prey produced as a recombinant fusion protein in HEK293F cell system.
Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cell system; prey produced as a recombinant fusion protein in HEK293F cell system.
