FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sumo, GluProRS
FlyBase ID
FBig0000121529
Interaction Network
Interactions Browser links
Sumo network
GluProRS network
Reported Interactions
FBrf0208144-1.coIP.MS
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

His-Flag tag

GluProRS 

EPRS

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A two step coIP of Sumo was utilized (His followed by Flag).

(Nie et al., 2009)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nie et al., 2009)
FBrf0208144-2.PD.WB
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

HIS tag

GluProRS 

EPRS

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nie et al., 2009)

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nie et al., 2009)
FBrf0251600-9.PD.WB
Reference
Nayak et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
GluProRS 

GluProRS

bait

GST tag

Sumo 

Sumo

prey

His tag

Sumo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
C-terminal double glycine motif residues 87-88
necessary binding region
aa 87-88

coordinates relative to Sumo-PA; deltaGG mutant is unable to conjugate to a substrate.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nayak et al., 2021)

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nayak et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (2)