FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AspRS, Sumo
FlyBase ID
FBig0000121642
Interaction Network
Interactions Browser links
AspRS network
Sumo network
Reported Interactions
FBrf0251600-11.PD.WB
Reference
Nayak et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AspRS 

AspRS

bait

HA tag

AspRS 
Sumo 

Sumo

prey

His tag

Sumo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
C-terminal double glycine motif residues 87-88
necessary binding region
aa 87-88

coordinates relative to Sumo-PA

AspRS 
interaction domain residues 147 and 383
necessary binding region
aa 147 and 383

K147R, K383R; coordinates relative to AspRS-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nayak et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nayak et al., 2021)
FBrf0251600-5.PD.WB
Reference
Nayak et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AspRS 

AspRS

bait

GST tag

Sumo 

Sumo

prey

His tag

Sumo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
C-terminal double glycine motif residues 87-88
necessary binding region
aa 87-88

coordinates relative to Sumo-PA; deltaGG mutant is unable to conjugate to a substrate.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nayak et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nayak et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)