FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Chro, M1BP
FlyBase ID
FBig0000121701
Interaction Network
Interactions Browser links
Chro network
M1BP network
Reported Interactions
FBrf0253430-29.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait

GFP tag

Chro 
M1BP 

M1BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
C terminal domain
sufficient binding region
aa 613-926

coordinates relative to Chro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
FBrf0262154-14.coIP.WB
Reference
Osadchiy et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

bait

FLAG tag

Chro 

Chro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Osadchiy et al., 2025)
FBrf0262154-6.Y2H
Reference
Osadchiy et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait

fused to GAL4 DNA-binding domain

M1BP 

M1BP

prey

fused to GAL4 activation domain

M1BP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
interaction domain
necessary binding region
aa 110-195

coordinates relative to M1BP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Osadchiy et al., 2025)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Osadchiy et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (2)