FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sumo, 14-3-3ζ
FlyBase ID
FBig0000121709
Interaction Network
Interactions Browser links
Sumo network
14-3-3ζ network
Reported Interactions
FBrf0208144-7.coIP.MS
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

His-Flag tag

14-3-3ζ 

14-3-3ζ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A two step coIP of Sumo was utilized (His followed by Flag).

(Nie et al., 2009)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nie et al., 2009)
FBrf0208144-8.PD.WB
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

HIS tag

14-3-3ζ 

14-3-3ζ

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nie et al., 2009)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nie et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (1)