C1703A, C1706A; coordinates relative to Ubr1-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Starvation or re-feeding free amino acids to medium induced interaction of N and C terminal regions of Ubr1 (FigS7D). Interaction disrupted in fed state. Supplement of effective amino acids (Arg, His, Leu, Lys, Phe and Trp), but not other amino acids (Met, Thr and Val) in the ice-cold, detergent-extracted lysates of starved S2 cells, suppressed the auto-inhibitory interaction of Ubr1 (FigS7E).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction between N and C terminal regions of Ubr1 increased with increasing time of starvation.
G157D and D160E; coordinates relative to Ubr1-PA
D244N and H247Y; coordinates relative to Ubr1-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
