FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Med, Ote
FlyBase ID
FBig0000121762
Interaction Network
Interactions Browser links
Med network
Ote network
Reported Interactions
FBrf0204627-1.FRET.FM
Reference
Jiang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ote 

Ote

fluorescence donor

CFP tag

Ote 

Ote

fluorescence donor

CFP tag

Ote 

Ote

fluorescence donor

CFP tag

Med 

Med

fluorescence acceptor

YFP tag

Med 

Med

fluorescence acceptor

YFP tag

Med 

Med

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Jiang et al., 2008)
FBrf0204627-2.coIP.WB
Reference
Jiang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ote 

Ote

bait
Med 

Med

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ote coimmunoprecipitates Med but not Mad.

(Jiang et al., 2008)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Jiang et al., 2008)
FBrf0204627-3.coIP.WB
Reference
Jiang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Med 

Med

bait

FLAG tag

Ote 

Ote

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jiang et al., 2008)
FBrf0204627-4.coIP.WB
Reference
Jiang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ote 

Ote

bait

FLAG tag

Ote 
Ote 
Med 

Med

prey

Myc tag

Med 
Med 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ote 
LEM domain
necessary binding region
aa 1-50

"ΔLEM" construct, coordinates relative to Ote-PA

Ote 
C-terminal region
necessary binding region
aa 271-400

"Δ271-400" construct, coordinates relative to Ote-PA

Med 
MH1 domain and linker region
sufficient binding region
Med 
linker region
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (1)