FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Clamp
FlyBase ID
FBig0000121765
Interaction Network
Interactions Browser links
Clamp network
Reported Interactions
FBrf0253164-2.Y2H
Reference
Tikhonova et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clamp 

CLAMP

bait

fused to GAL4 DNA-binding domain

Clamp 

CLAMP

prey

fused to GAL4 activation domain

Clamp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clamp 
dimerization domain residues 46-86
necessary binding region
aa 46-86

coordinates relative to Clamp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tikhonova et al., 2022)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Tikhonova et al., 2022)
FBrf0253164-3.CH.MW
Reference
Tikhonova et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clamp 

CLAMP

self

thioredoxin tag

Clamp 

CLAMP

self

thioredoxin tag

Clamp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clamp 
dimerization domain residues 41-91
sufficient binding region
aa 41-91

coordinates relative to Clamp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Results support Y2H results of aa 46-86 for homo-dimerization of Clamp.

(Tikhonova et al., 2022)

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

(Tikhonova et al., 2022)
FBrf0253164-4.PD.MW
Reference
Tikhonova et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clamp 

CLAMP

bait

GST tag

Clamp 

CLAMP

prey

His thioredoxin tag

Clamp 
Clamp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clamp 
dimerization domain residues 1-86
necessary binding region
aa 1-86

Deletion construct within the context of 153 aa construct; coordinates relative to Clamp-PB.

Clamp 
dimerization domain residues 41-113
sufficient binding region
aa 41-113

coordinates relative to Clamp-PB.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Deletion constructs tested with GST tagged Clamp 1-113 aa construct. Results support Y2H results of aa 46-86 for homo-dimerization of Clamp.

(Tikhonova et al., 2022)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tikhonova et al., 2022)
FBrf0253164-5.XS
Reference
Tikhonova et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clamp 

CLAMP

self
Clamp 

CLAMP

self
Clamp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clamp 
dimerization domain
sufficient binding region
aa 1-113

coordinates relative to Clamp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Clamp forms a homodimer.

(Tikhonova et al., 2022)

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Tikhonova et al., 2022)
FBrf0253786-1.Y2H
Reference
Tikhonova et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clamp 

CLAMP

bait

fused to GAL4 DNA-binding domain

Clamp 

CLAMP

prey

fused to GAL4 activation domain

Clamp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clamp 
zinc finger domain
sufficient binding region
aa 1-153
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tikhonova et al., 2022)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control

(Tikhonova et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)