FlyBase Gene Report: Dmel\Clamp

General Information

Symbol

Dmel\Clamp

Species

D. melanogaster

Name

Chromatin-linked adaptor for MSL proteins

Annotation Symbol

CG1832

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032979

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Chromatin-linked adaptor for MSL proteins (Clamp) encodes a zinc finger protein that is required to recruit the dosage compensation complex. It is essential in both males and females. It also functions outside of the dosage compensation pathway. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

40F2-40F2

Sequence location

2L:22,165,720..22,169,143 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:22165720..22169143

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from direct assay

(Bag et al., 2019)

enables chromatin-protein adaptor activity

inferred from direct assay

(Soruco et al., 2013)

enables DNA binding

inferred from direct assay

(Soruco et al., 2013)

inferred from mutant phenotype

(Tikhonova et al., 2019)

enables DNA-binding transcription activator activity, RNA polymerase II-specific

inferred from mutant phenotype

(Rieder et al., 2017)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from direct assay

(Duan et al., 2021)

enables RNA polymerase II transcription regulatory region sequence-specific DNA binding

inferred from direct assay

(Rieder et al., 2017)

inferred from direct assay

(Kuzu et al., 2016)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from direct assay

(Colonnetta et al., 2021, Duan et al., 2021)

inferred from mutant phenotype

(Rieder et al., 2017)

involved_in dosage compensation by hyperactivation of X chromosome

inferred from direct assay

(Colonnetta et al., 2021, Kuzu et al., 2016, Soruco et al., 2013)

involved_in maternal-to-zygotic transition of gene expression

inferred from direct assay

(Colonnetta et al., 2021, Duan et al., 2021)

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Rieder et al., 2017)

involved_in regulation of gene expression

inferred from mutant phenotype

(Soruco et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

is_active_in chromatin

inferred from direct assay

(Urban et al., 2017)

located_in chromatin

inferred from direct assay

(Bag et al., 2019)

located_in chromosome

inferred from direct assay

(Soruco et al., 2013)

inferred from mutant phenotype

(Tikhonova et al., 2019)

is_active_in histone locus body

inferred from direct assay

(Rieder et al., 2017)

located_in nucleus

inferred from direct assay

(Bag et al., 2019)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Protein Function (UniProtKB)

Transcription factor involved in X-chromosome dosage compensation in males, the process by which transcription of the single X chromosome in the male is elevated (PubMed:23873939, PubMed:27414415, PubMed:27995349, PubMed:31320325, PubMed:34187599, PubMed:37602401). Binds to the DNA sequence (GA)n (PubMed:27414415, PubMed:28838946, PubMed:29281702). Clamp-binding promotes nucleosome depletion and chromatin accessibility, thereby allowing access to other transcription factors (PubMed:28838946, PubMed:29077765, PubMed:34187599). Specifically binds to cis-acting elements on the X-chromosome named chromatin entry sites and promotes recruitment of the male-specific lethal (MSL) histone acetyltransferase complex, which associates with actively transcribed genes on the male X-chromosome to upregulate their expression (PubMed:23873939, PubMed:27414415, PubMed:31320325). Mechanistically, acts by promoting chromatin accessibility at chromatin entry sites, facilitating DNA-binding of msl-2, followed by MSL complex recruitment (PubMed:31320325, PubMed:37602401). In addition to dosage compensation, also involved in zygotic genome activation (ZGA), a critical event in early embryonic development during which the developmental control passes from maternally provided mRNAs to the expression of the zygotic genome after fertilization (PubMed:34342574, PubMed:34849887). Maternally-provided protein cooperates with Zelda (zld) to activate zygotic transcription by increasing chromatin accessibility at promoters of specific genes and facilitate zld occupancy at a subset of key embryonic promoters (PubMed:34342574). Also acts as an activator of gypsy chromatin insulator function by promoting binding of Cp190 to chromatin (PubMed:30718365).

(UniProt, Q9V9N4)

Summary (Interactive Fly)

zinc finger transcription factor - dosage compensation - essential in both males and females - also functions outside of the dosage compensation pathway

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Clamp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V9N4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

7NF9

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Clamp-RA

FBtr0085964

NM_136293

2636

561

Clamp-RB

FBtr0100407

NM_001014498

2897

566

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Clamp-PA

FBpp0085317

61.2

561

9.06

FBpp0099821

61.8

566

9.02

Q8IGP5

NP_001014498

AAX52677

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer (PubMed:35409222). Interacts with msl-2; promoting recruitment of the male-specific lethal (MSL) histone acetyltransferase complex to chromatin (PubMed:31320325, PubMed:35648444, PubMed:37602401). Interacts with Nelf-A (PubMed:29281702). Interacts with NELF-B (PubMed:29281702).

(UniProt, Q9V9N4)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

7NF9

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V9N4

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Clamp using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.78

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in chromatin

inferred from direct assay

(Urban et al., 2017)

located_in chromatin

inferred from direct assay

(Bag et al., 2019)

located_in chromosome

inferred from direct assay

(Soruco et al., 2013)

inferred from mutant phenotype

(Tikhonova et al., 2019)

is_active_in histone locus body

inferred from direct assay

(Rieder et al., 2017)

located_in nucleus

inferred from direct assay

(Bag et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Clamp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Clamp

View exonic expression by tissue for Dmel\Clamp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032979

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35445/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032979

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032979

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032979

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1832

Flygut - An atlas of the Drosophila adult midgut

FBgn0032979

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Clamp

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Clamp

Transgenic constructs containing regulatory region of Clamp

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | male limited, with Scer\GAL4^Bx-MS1096

Clamp^{UAS.ORF.GW.Tag:HA}

lethal - all die before end of P-stage | recessive

Clamp²

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Clamp^GD9558

viable, with Scer\GAL4^Mef2.PR

Clamp^GD9558

viable, with Scer\GAL4^pnr-MD237

Clamp^GD9558

Other Phenotypes

Allele

abnormal developmental rate | larval stage

Clamp²

some die during larval stage

Clamp²

visible | female limited, with Scer\GAL4^Bx-MS1096

Clamp^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

polytene chromosome | larval stage

Clamp²

wing | female limited, with Scer\GAL4^Bx-MS1096

Clamp^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (290)

Hsap\ZNF25

2 of 14

Yes

Hsap\ZNF75A

2 of 14

Yes

Hsap\ZNF77

2 of 14

Yes

Hsap\ZNF79

2 of 14

Yes

Hsap\ZNF133

2 of 14

Yes

Hsap\ZNF169

2 of 14

Yes

Hsap\ZNF337

2 of 14

Yes

Hsap\ZNF398

2 of 14

Yes

Hsap\ZNF425

2 of 14

Yes

Hsap\ZNF436

2 of 14

Yes

Hsap\ZNF554

2 of 14

Yes

Hsap\ZNF559

2 of 14

Yes

Hsap\ZNF582

2 of 14

Yes

Hsap\ZNF596

2 of 14

Yes

Hsap\ZNF674

2 of 14

Yes

Hsap\ZNF749

2 of 14

Yes

Hsap\ZSCAN2

2 of 14

Yes

Hsap\BCL6B

1 of 14

Yes

Hsap\BCL6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC344065

1 of 14

Hsap\LOC728743

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZBTB7C

1 of 14

Yes

Hsap\ZBTB14

1 of 14

Hsap\ZBTB16

1 of 14

Yes

Hsap\ZBTB17

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF35

1 of 14

Hsap\ZNF37A

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF239

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF354C

1 of 14

Hsap\ZNF358

1 of 14

Hsap\ZNF366

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF396

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF415

1 of 14

Hsap\ZNF419

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF506

1 of 14

Hsap\ZNF510

1 of 14

Hsap\ZNF517

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF552

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\ZNF660-ZNF197

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF737

1 of 14

Hsap\ZNF766

1 of 14

Yes

Hsap\ZNF768

1 of 14

Hsap\ZNF773

1 of 14

Yes

Hsap\ZNF778

1 of 14

Hsap\ZNF782

1 of 14

Hsap\ZNF784

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZSCAN5B

1 of 14

Yes

Hsap\ZSCAN5C

1 of 14

Hsap\ZSCAN5DP

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (84)

Rnor\Zfp90

2 of 14

Yes

Rnor\Bcl6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\LOC108348125

1 of 14

Yes

Rnor\Prdm10

1 of 14

Yes

Rnor\RGD1564807

1 of 14

Rnor\RGD1566386

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Vom1r42

1 of 14

Yes

Rnor\Zbtb6

1 of 14

Yes

Rnor\Zbtb7a

1 of 14

Yes

Rnor\Zbtb7c

1 of 14

Yes

Rnor\Zbtb17

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp84

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp133

1 of 14

Rnor\Zfp136

1 of 14

Yes

Rnor\Zfp157

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp251

1 of 14

Yes

Rnor\Zfp267

1 of 14

Rnor\Zfp282

1 of 14

Yes

Rnor\Zfp329

1 of 14

Rnor\Zfp366

1 of 14

Yes

Rnor\Zfp386

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp617

1 of 14

Yes

Rnor\Zfp663

1 of 14

Yes

Rnor\Zfp707l1

1 of 14

Yes

Rnor\Zfp707

1 of 14

Rnor\Zfp709

1 of 14

Rnor\Zfp717

1 of 14

Yes

Rnor\Zfp763

1 of 14

Yes

Rnor\Zfp777

1 of 14

Yes

Rnor\Zfp949

1 of 14

Yes

Rnor\Zfp952

1 of 14

Yes

Rnor\Zfp955a

1 of 14

Yes

Rnor\Zkscan8

1 of 14

Yes

Rnor\Znf442

1 of 14

Yes

Rnor\Zscan4f

1 of 14

Rnor\Zscan22

1 of 14

Yes

Mus musculus (laboratory mouse) (135)

Mmus\Gm10033

2 of 14

Yes

Mmus\Zfp90

2 of 14

Yes

Mmus\Zfp329

2 of 14

Yes

Mmus\2610008E11Rik

1 of 14

Mmus\2810021J22Rik

1 of 14

Mmus\A430033K04Rik

1 of 14

Yes

Mmus\AI987944

1 of 14

Mmus\AU041133

1 of 14

Mmus\Bcl6b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm3604

1 of 14

Mmus\Gm3854

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm14412

1 of 14

Mmus\Gm14430

1 of 14

Yes

Mmus\Gm14434

1 of 14

Mmus\Gm21994

1 of 14

Yes

Mmus\Gm45871

1 of 14

Mmus\Ikzf1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zbtb7a

1 of 14

Yes

Mmus\Zbtb7c

1 of 14

Yes

Mmus\Zbtb14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp57

1 of 14

Yes

Mmus\Zfp61

1 of 14

Mmus\Zfp62

1 of 14

Mmus\Zfp65

1 of 14

Yes

Mmus\Zfp69

1 of 14

Yes

Mmus\Zfp81

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp266

1 of 14

Mmus\Zfp267

1 of 14

Mmus\Zfp282

1 of 14

Yes

Mmus\Zfp316

1 of 14

Mmus\Zfp322a

1 of 14

Yes

Mmus\Zfp366

1 of 14

Yes

Mmus\Zfp386

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp777

1 of 14

Yes

Mmus\Zfp790

1 of 14

Yes

Mmus\Zfp791

1 of 14

Mmus\Zfp808

1 of 14

Mmus\Zfp809

1 of 14

Yes

Mmus\Zfp810

1 of 14

Mmus\Zfp882

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp955a

1 of 14

Yes

Mmus\Zfp955b

1 of 14

Yes

Mmus\Zfp960

1 of 14

Mmus\Zfp961

1 of 14

Yes

Mmus\Zfp971

1 of 14

Mmus\Zim1

1 of 14

Yes

Mmus\Zkscan3

1 of 14

Mmus\Zkscan4

1 of 14

Yes

Mmus\Zkscan8

1 of 14

Yes

Mmus\Zscan4b

1 of 14

Yes

Mmus\Zscan4c

1 of 14

Yes

Mmus\Zscan4d

1 of 14

Yes

Mmus\Zscan4e

1 of 14

Yes

Mmus\Zscan4f

1 of 14

Yes

Mmus\Zscan4-ps1

1 of 14

Yes

Mmus\Zscan4-ps2

1 of 14

Yes

Mmus\Zscan4-ps3

1 of 14

Yes

Mmus\Zscan10

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (68)

Xtro\LOC101733761

2 of 13

Yes

Xtro\znf585a

2 of 13

Yes

Xtro\adprm.2

1 of 13

Xtro\cxcl9

1 of 13

Yes

Xtro\e4f1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ikzf3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\mynn

1 of 13

Xtro\parp6

1 of 13

Yes

Xtro\plag1

1 of 13

Xtro\prdm1

1 of 13

Xtro\prdm6

1 of 13

Yes

Xtro\prdm10

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\xlcgf26.1l

1 of 13

Yes

Xtro\zbtb7c

1 of 13

Yes

Xtro\zbtb14

1 of 13

Yes

Xtro\zbtb18

1 of 13

Yes

Xtro\zbtb25

1 of 13

Yes

Xtro\zfat

1 of 13

Xtro\zfp2

1 of 13

Xtro\znf81

1 of 13

Yes

Xtro\znf229

1 of 13

Xtro\znf276

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (62)

Drer\bcl6b

1 of 14

Yes

Drer\drll.3

1 of 14

Yes

Drer\egr1

1 of 14

Yes

Drer\hic1

1 of 14

Yes

Drer\ikzf1

1 of 14

Yes

Drer\ikzf2

1 of 14

Yes

Drer\ikzf5

1 of 14

Yes

Drer\klf5l

1 of 14

Yes

Drer\klf9

1 of 14

Yes

Drer\klf11a

1 of 14

Yes

Drer\klf11b

1 of 14

Yes

Drer\LOC568955

1 of 14

Yes

Drer\LOC797855

1 of 14

Yes

Drer\LOC100329790

1 of 14

Yes

Drer\LOC100332458

1 of 14

Yes

Drer\LOC101883230

1 of 14

Yes

Drer\LOC108184006

1 of 14

Yes

Drer\LOC110437792

1 of 14

Yes

Drer\LOC110439308

1 of 14

Yes

Drer\LOC110439430

1 of 14

Yes

Drer\osr2

1 of 14

Yes

Drer\sall1a

1 of 14

Yes

Drer\sall4

1 of 14

Yes

Drer\scrt1a

1 of 14

Yes

Drer\scrt1b

1 of 14

Yes

Drer\si:cabz01021433.2

1 of 14

Yes

Drer\si:cabz01069020.1

1 of 14

Yes

Drer\si:ch73-266f23.3

1 of 14

Yes

Drer\si:ch211-198a12.6

1 of 14

Yes

Drer\si:ch211-222k6.1

1 of 14

Yes

Drer\si:ch211-229l10.11

1 of 14

Yes

Drer\si:ch1073-224n8.1

1 of 14

Yes

Drer\si:dkey-14d8.1

1 of 14

Yes

Drer\si:dkey-34m19.3

1 of 14

Yes

Drer\si:dkey-56e3.3

1 of 14

Yes

Drer\si:dkey-82i20.2

1 of 14

Yes

Drer\si:dkey-154p10.3

1 of 14

Yes

Drer\si:dkey-237m9.1

1 of 14

Yes

Drer\si:dkeyp-53d3.6

1 of 14

Yes

Drer\snai3

1 of 14

Yes

Drer\sp2

1 of 14

Yes

Drer\sp3a

1 of 14

Yes

Drer\sp5a

1 of 14

Yes

Drer\sp5b

1 of 14

Yes

Drer\sp5l

1 of 14

Yes

Drer\zbtb7c

1 of 14

Yes

Drer\zbtb16a

1 of 14

Yes

Drer\zbtb33

1 of 14

Yes

Drer\zeb2b

1 of 14

Yes

Drer\zgc:113209

1 of 14

Yes

Drer\zgc:113220

1 of 14

Yes

Drer\zgc:171435

1 of 14

Yes

Drer\znf148

1 of 14

Yes

Drer\znf341

1 of 14

Yes

Drer\znf408

1 of 14

Yes

Drer\znf513a

1 of 14

Yes

Drer\znf574

1 of 14

Yes

Drer\znf592

1 of 14

Yes

Drer\znf740a

1 of 14

Yes

Drer\znf865

1 of 14

Yes

Drer\znf1015

1 of 14

Yes

Drer\znf1068

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\bcl-11

1 of 14

Yes

Cele\egrh-3

1 of 14

Yes

Cele\hinf-1

1 of 14

Yes

Cele\klf-2

1 of 14

Yes

Cele\klu-1

1 of 14

Yes

Cele\klu-2

1 of 14

Yes

Cele\row-1

1 of 14

Yes

Cele\znf-782

1 of 14

Yes

Cele\ztf-14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (46)

Agam\AgaP_AGAP008827

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP004269

1 of 12

Agam\AgaP_AGAP004331

1 of 12

Agam\AgaP_AGAP004764

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP006794

1 of 12

Agam\AgaP_AGAP007389

1 of 12

Agam\AgaP_AGAP007804

1 of 12

Yes

Agam\AgaP_AGAP008232

1 of 12

Agam\AgaP_AGAP008616

1 of 12

Yes

Agam\AgaP_AGAP008984

1 of 12

Agam\AgaP_AGAP009010

1 of 12

Agam\AgaP_AGAP009482

1 of 12

Yes

Agam\AgaP_AGAP009483

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012669

1 of 12

Agam\AgaP_AGAP012834

1 of 12

Yes

Agam\AgaP_AGAP013293

1 of 12

Agam\ODD_a

1 of 12

Agam\ODD_b

1 of 12

Arabidopsis thaliana (thale-cress) (13)

Atha\AT1G14580

1 of 13

Yes

Atha\AT3G45260

1 of 13

Yes

Atha\AT5G60470

1 of 13

Yes

Atha\IDD1

1 of 13

Yes

Atha\IDD2

1 of 13

Yes

Atha\IDD4

1 of 13

Yes

Atha\IDD5

1 of 13

Yes

Atha\IDD7

1 of 13

Yes

Atha\IDD11

1 of 13

Yes

Atha\JKD

1 of 13

Yes

Atha\MGP

1 of 13

Yes

Atha\NUC

1 of 13

Yes

Atha\YY1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\CMR3

1 of 13

Yes

Scer\YPR015C

1 of 13

Yes

Scer\ZAP1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Clamp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (62)

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

klu

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Cf2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

hkb

1 of 13

idc

1 of 13

Kr-h1

1 of 13

M1BP

1 of 13

MTF-1

1 of 13

odd

1 of 13

Opbp

1 of 13

pad

1 of 13

pita

1 of 13

Plzf

1 of 13

prg

1 of 13

ranshi

1 of 13

row

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

sna

1 of 13

sqz

1 of 13

Sry-β

1 of 13

Sry-δ

1 of 13

su(Hw)

1 of 13

sug

1 of 13

trem

1 of 13

wor

1 of 13

Zif

1 of 13

zld

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Clamp

two hybrid, pull down, molecular weight estimation by staining, x ray scattering, predetermined participant, molecular sieving, cross-linking study

(Tikhonova et al., 2022, Tikhonova et al., 2022)

Clamp - CG10321

two hybrid array

(Shokri et al., 2019)

Clamp - Cp190

anti bait coimmunoprecipitation, western blot

(Bag et al., 2019)

Clamp - Deaf1

two hybrid, two hybrid array

(Shokri et al., 2019, Hu et al., 2017.6.13)

Clamp - Eip78C

two hybrid array

(Shokri et al., 2019)

Clamp - Fmr1

anti bait coimmunoprecipitation, western blot

(Ray et al., 2023)

Clamp - Hr78

two hybrid array

(Shokri et al., 2019)

Clamp - NELF-B

anti bait coimmunoprecipitation, western blot

(Urban et al., 2017)

Clamp - Nelf-A

anti bait coimmunoprecipitation, western blot

(Urban et al., 2017)

Clamp - Nf-YC

two hybrid

(Hu et al., 2017.6.13)

Clamp - mle

proximity ligation assay, fluorescence microscopy, two hybrid, anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Tikhonova et al., 2024, Lindehell et al., 2015)

Clamp - mod(mdg4)

anti bait coimmunoprecipitation, western blot

(Bag et al., 2019)

Clamp - msl-3

anti tag coimmunoprecipitation, peptide massfingerprinting, proximity ligation assay, fluorescence microscopy

(Zee et al., 2016, Lindehell et al., 2015, Alekseyenko et al., 2014)

Clamp - su(Hw)

anti bait coimmunoprecipitation, western blot

(Bag et al., 2019)

RNA-protein

Physical Interaction

Assay

References

Clamp - lncRNA:roX1

pull down, western blot

(Quinn et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9V9N4 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61385

DroID - A comprehensive database of gene and protein interactions.

FBgn0032979

MIST (protein-protein) - An integrated Molecular Interaction Database

35445

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

40F2-40F2

Sequence location

2L:22,165,720..22,169,143 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

40F2-40F2

Limits computationally determined from genome sequence between P{lacW}Df31^k05815 and P{PZ}cul-2⁰²⁰⁷⁴&P{lacW}l(2)k08110^k08110

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

66395

w¹¹¹⁸; PBac{Clamp-GFP.FPTB}VK00033

27080

y¹ v¹; P{TRiP.JF02426}attP2

14352

y¹; P{SUPor-P}Clamp^KG00473/SM6a; ry⁵⁰⁶

FlyORF

F002203

M{UAS-Clamp.ORF.3xHA.GW}ZH-86Fb

F004898

M{UAS-Clamp.ORF-VNshort}ZH-86Fb

NIG-Fly

1832R-2

P{NIG.1832R}2

M2L-0445

y¹ w¹¹¹⁸; Clamp^SK1 P{neoFRT}40A/CyO-Tb

M2L-0446

y¹ w¹¹¹⁸; Clamp^SK2 P{neoFRT}40A/CyO-Tb

VDRC

v108039

P{KK106004}VIE-260B

v25316

w¹¹¹⁸; P{GD9558}v25316

v318550

PBac{fTRG00362.sfGFP-TVPTBF}VK00033

v342333

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02117}attP40/CyO-GFP

More stocks available...

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (106)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032979

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032979

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Tikhonova et al., 2019, Soruco et al., 2013, Larschan et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Clamp CG1832

(Soruco et al., 2013)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Clamp

(Jans et al., 2024, Makki and Meller, 2024, Nieken et al., 2023, Tang et al., 2023, Zhao et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Sebastian et al., 2022, Peterson et al., 2021, Witt et al., 2021, Meyer-Nava et al., 2020, Kyrchanova et al., 2019, Prayitno et al., 2019, Shokri et al., 2019, Hu et al., 2017.6.13, Rieder et al., 2017, Schertel et al., 2015)

clamp

(Colonnetta et al., 2023, Duan et al., 2021, Koreski et al., 2020, Bischof et al., 2018, Kaye et al., 2017, Urban et al., 2017, Urban et al., 2017)

Name Synonyms

CLAMP

(Hunt et al., 2024, Eggers et al., 2023)

Chromatin-linked adaptor for MSL proteins

Clamp

(Kyrchanova et al., 2023)

chromatin-linked adaptor for MSL proteins

(McElroy et al., 2014, Soruco et al., 2013)

Secondary FlyBase IDs

FBan0001832

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35445

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V9N4

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V9N4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V9N4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032979

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35445/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032979

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032979

FlyMine - An integrated database for Drosophila genomics

FBgn0032979

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1832

MARRVEL_MODEL - MARRVEL (model organism gene)

35445

PDB - An information portal to biological macromolecular structures

7NF9

Linkouts

BioGRID - A database of protein and genetic interactions.

61385

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032979

DroID - A comprehensive database of gene and protein interactions.

FBgn0032979

DRSC - Results frm RNAi screens

FBgn0032979

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032979

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032979

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1832

Flygut - An atlas of the Drosophila adult midgut

FBgn0032979

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015048

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

35445

References (109)

The following external sites may use different assemblies or annotations than FlyBase.

0.78

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