sryδ, serendipity delta, sry δ, EH8, sry
Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
The Sry-δ protein
was shown to specifically bind the "sdcs" consensus sequence in the bcd 5'
protmoter fragments were tested for their ability to drive expression in the
nurse cell specific expression.
Binds DNA as a homodimer. N-terminal regions of the protein are required, in addition to the zinc fingers, for the specificity of chromatin-binding.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sry-δ using the Feature Mapper tool.
During early oogenesis, the Sry-delta protein localizes to nurse and follicle cell nuclei. Close inspection of nurse cell nuclei indicates that Sry-delta protein is bound to chromatin. After stage S12 of oogenesis, the protein is found in the oocyte cytoplasm. During embryogenesis, the protein once again becomes nuclear.
During early oogenesis, the Sry-δ protein localizes to nurse and follicle cell nuclei. Close inspection of nurse cell nuclei indicates that Sry-δ protein is bound to chromatin. After stage S12 of oogenesis, the protein is found in the oocyte cytoplasm. During embryogenesis, the protein once again becomes nuclear.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Sry-δ in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Sry-δ protein is a sequence-specific transcriptional activator. A single Sry-δ protein consensus binding site (SDCS), in either orientation, is sufficient to promote transcription activation in cell culture.
The molecular organization of the 'rp49-Sry-jan' gene cluster, including gene order, direction of transcription and partial overlap of the janA and janB genes is strictly conserved between D.melanogaster and D.pseudoobscura.pseudoobscura.
The carboxy-terminal DNA binding finger domain is required and sufficient for binding at specific chromosomal sites but this binding does not nearly reproduce the wild type pattern. An amino-terminal domain is essential to its specificity of in vivo interaction with chromatin. In vitro and in vivo experiments using reciprocal finger swap between Sry-β and Sry-δ proteins suggests that the in vivo specificity is dependent on selective protein-protein contacts at defined chromosomal sites, in addition to DNA specific recognition.
P-element mediated transformation demonstrates that an 18 amino acid segment from the Sry-δ protein contains a nuclear localisation sequence able to direct Ecol\lacZ into the nucleus of all tissues examined. Sequence homology to the SV40 large T and human c-myc NLS suggests that the motif included in the 18 amino acid stretch could be a nuclear localisation signal.