FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wun2, Itgbn
FlyBase ID
FBig0000121782
Interaction Network
Interactions Browser links
wun2 network
Itgbn network
Reported Interactions
FBrf0255318-4.Y2H
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wun2 

Wun2

bait

fused to C-terminal ubiquitin

Itgbn 

β[v]integrin

prey

fused to N-terminal ubiquitin

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Gao et al., 2022)

Split ubiquitin yeast two hybrid system.

(Gao et al., 2022)
FBrf0255318-7.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wun2 

Wun2

bait

TurboID-Flag tag

wun2 
Itgbn 

β[v]integrin

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wun2 
noncatalytic domain
necessary binding region
aa 116-134

Deletion construct.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)

Proximity biotin labeling of wun2 interacting proteins by TurboID ligase with subsequent immunoprecipitation with biotin. Interaction with Itgbn identified with anti-GFP.

(Gao et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)