FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lst8, sau
FlyBase ID
FBig0000121798
Interaction Network
Interactions Browser links
Lst8 network
sau network
Reported Interactions
FBrf0255112-5.PD.WB
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lst8 

Lst8

bait

GST tag

sau 

dGOLPH3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild type testes extract.

(Frappaolo et al., 2022)
FBrf0255112-7.PD.WB
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lst8 

Lst8

bait

GST tag

sau 

dGOLPH3

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Frappaolo et al., 2022)
FBrf0255112-8.Y2H
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

dGOLPH3

bait

fused to LexA DNA-binding domain

Lst8 

Lst8

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Frappaolo et al., 2022)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Frappaolo et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)