Atg14, Miga Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Miga

bait

V5 tag

Miga

Atg14

prey

HA tag

Atg14

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Atg14

M domain

sufficient binding region

aa 209-443

coordinates relative to Atg14-PA

Atg14

CCD domain

sufficient binding region

aa 99-208

coordinates relative to Atg14-PA

Miga

N-terminal domain

sufficient binding region

aa 1-255

coordinates relative to Miga-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction occurred under both fed and starvation conditions; Miga could pull down both Atg14 and Vap33 when they were coexpressed.

(Xu et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)

FBrf0254939-11.coIP.WB

Reference

Xu et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Atg14

bait

GFP tag

Miga

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)

FBrf0254939-13.coIP.WB

Reference

Xu et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Atg14

bait

HA tag

Miga

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Immunoprecipitation of Miga by Atg14 disrupted when Atg6 was also expressed.

(Xu et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)

FBrf0254939-16.coIP.WB

Reference

Xu et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Miga

bait

Flag-HA tag

Atg14

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Miga could pull down both Atg14 and Uvrag when all three proteins were co-expressed.

(Xu et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)

FBrf0254939-2.coIP.WB

Reference

Xu et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used