FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rga, pum
FlyBase ID
FBig0000121801
Interaction Network
Interactions Browser links
Rga network
pum network
Reported Interactions
FBrf0254621-2.Y2H
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pumillo

bait

fused to GAL4 DNA-binding domain

pum 
pum 
pum 
Rga 

Not2

prey

fused to GAL4 activation domain

Rga 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RD3 domain
sufficient binding region
aa 847-1091
pum 
RD3 domain residues 1033 and 1040
mutation decreasing interaction
aa 1033 and 1040

F1033A, F1040A

pum 
RD3 (CR1 and CR4) domain
mutation decreasing interaction
aa 893-1037

deletion construct

Rga 
Not-box domain
sufficient binding region
aa 465-585
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Haugen et al., 2022)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Haugen et al., 2022)
FBrf0254621-6.coIP.WB
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rga 

Not2

bait

Flag tag

pum 

pum

prey

V5 tag

pum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RD3 domain
sufficient binding region
aa 847-1091
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haugen et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)