FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Imp, lin-28
FlyBase ID
FBig0000121805
Interaction Network
Interactions Browser links
Imp network
lin-28 network
Reported Interactions
FBrf0254532-3.Y2H
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lin-28 

Lin28

bait

fused to LexA DNA-binding domain

Imp 

Imp

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Sreejith et al., 2022)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sreejith et al., 2022)
FBrf0254532-4.Y2H
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

fused to LexA DNA-binding domain

lin-28 

Lin28

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Sreejith et al., 2022)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sreejith et al., 2022)
FBrf0254532-5.coIP.WB
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

FLAG tag

lin-28 

Lin28

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sreejith et al., 2022)
FBrf0254532-6.coIP.WB
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
lin-28 

lin28

bait

HA tag

Imp 

Imp

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sreejith et al., 2022)

Interaction in RNA independent.

(Sreejith et al., 2022)
FBrf0254532-7.BiFC.FM
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

GFP tag, N-terminal fragment

lin-28 

Lin28

prey

GFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293T cells; proteins produced from transfected constructs.

(Sreejith et al., 2022)
FBrf0254532-8.BiFC.FM
Reference
Sreejith et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lin-28 

Lin28

bait

GFP tag, N-terminal fragment

Imp 

Imp

prey

GFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293T cells; proteins produced from transfected constructs.

(Sreejith et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)