FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
BEAF-32, polybromo
FlyBase ID
FBig0000121840
Interaction Network
Interactions Browser links
BEAF-32 network
polybromo network
Reported Interactions
FBrf0254954-1.coIP.MS
Reference
McKowen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait

Flag tag

polybromo 

polybromo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(McKowen et al., 2022)
FBrf0254954-2.Y2H
Reference
McKowen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait

fused to GAL4 DNA-binding domain

BEAF-32 
polybromo 

polybromo

prey

fused to GAL4 activation domain

polybromo 
polybromo 
polybromo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polybromo 
Bromodomains 4 and 5
sufficient binding region
aa 500-750

coordinates relative to polybromo-PA

polybromo 
Bromodomains 3 and 4
sufficient binding region
aa 327-635

coordinates relative to polybromo-PA

polybromo 
Bromodomains 5 and 6
sufficient binding region
aa 630-914

coordinates relative to polybromo-PA

BEAF-32 
Mid-LZ domains
sufficient binding region
aa 81-227
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD; polybromo bromodomains 4 and 5 interact with BEAF-32 Mid-LZ domains.

(McKowen et al., 2022)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(McKowen et al., 2022)
FBrf0254954-3.PD.WB
Reference
McKowen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait

Flag tag

polybromo 

polybromo

prey

Myc tag

polybromo 
polybromo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polybromo 
Bromodomains 4 and 5
sufficient binding region
aa 500-750

coordinates relative to polybromo-PA

polybromo 
Bromodomains 3 and 4
sufficient binding region
aa 327-635

coordinates relative to polybromo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(McKowen et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)