Split ubiquitin yeast two hybrid system.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

wun2 RC mutant replaced aa 155-177 with aa 345-350 and resulted in loss of interaction with Rip11.