FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
IntS8, Pp2A-29B
FlyBase ID
FBig0000121940
Interaction Network
Interactions Browser links
IntS8 network
Pp2A-29B network
Reported Interactions
FBrf0247102-22.coIP.MS
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS8 

IntS8

bait

FLAG tag

Pp2A-29B 

PR65

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
FBrf0247102-52.coIP.WB
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS8 

IntS8

bait

FLAG tag

IntS8 
Pp2A-29B 

PR65

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS8 
N-terminal domain residues 19-22
mutation disrupting interaction
aa 19-22

WFEF to AAAA, coordinates relative to IntS8-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
FBrf0247102-61.Y2H
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pp2A-29B 

PR65

bait

fused to GAL4 DNA-binding domain

Pp2A-29B 
IntS8 

IntS8

prey

fused to GAL4 activation domain

IntS8 
IntS8 
IntS8 
IntS8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pp2A-29B 
HEAT repeats 1-7
sufficient binding region
aa 1-287

coordinates relative to Pp2A-29B-PA

IntS8 
N-terminal domain
necessary binding region
aa 1-18

coordinates relative to IntS8-PA

IntS8 
N-terminal domain residues 15-18
mutation decreasing interaction
aa 15-18

ETVL to AAAA, coordinates relative to IntS8-PA

IntS8 
N-terminal domain residues 19-22
mutation disrupting interaction
aa 19-22

WFEF to AAAA, coordinates relative to IntS8-PA

IntS8 
N-terminal domain residues 23-26
mutation disrupting interaction
aa 23-26

LLDP to AAAA, coordinates relative to IntS8-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Huang et al., 2020)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Huang et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)