FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Chc, tacc
FlyBase ID
FBig0000121957
Interaction Network
Interactions Browser links
Chc network
tacc network
Reported Interactions
FBrf0253939-1.Y2H
Reference
Vazquez-Pianzola et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chc 

Chc

bait

fused to GAL4 DNA-binding domain

Chc 
tacc 

DTACC

prey

fused to GAL4 activation domain

tacc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chc 
interaction domain
sufficient binding region
aa 329-803
tacc 
Phosphorylation residue 863
mutation increasing interaction
aa 863

Phosphomimetic mutation S863D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Vazquez-Pianzola et al., 2022)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Vazquez-Pianzola et al., 2022)
FBrf0253939-5.coIP.WB
Reference
Vazquez-Pianzola et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chc 

Chc

bait

Myc tag

tacc 

D-TACC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)