Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.

Two-step coIP demonstrating IntS8 precipitates Dad and tkv in the first step. The first IP reactions was used for a second Dad coIP which demonstrated an interaction with tkv and IntS8 suggesting all three proteins associate.

Two-step coIP demonstrating IntS8 precipitates Dad and tkv in the first step. The first IP reactions was used for a second Dad coIP which demonstrated an interaction with tkv and IntS8, suggesting all three proteins associate.

Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.