FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4E3, me31B
FlyBase ID
FBig0000121992
Interaction Network
Interactions Browser links
eIF4E3 network
me31B network
Reported Interactions
FBrf0255814-2.Y2H
Reference
Layana et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E3 

eIF4E-3

bait

fused to GAL4 DNA-binding domain

eIF4E3 
me31B 

Me31B

prey

fused to GAL4 activation domain

me31B 
me31B 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E3 
interaction residue 103
mutation disrupting interaction
aa 103

coordinates relative to eIF4E3-PA

me31B 
D1 domain residues 1-96
sufficient binding region
aa 1-96
me31B 
interaction residue 63
mutation disrupting interaction
aa 63

F63A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Layana et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Layana et al., 2023)
FBrf0255814-5.FRET.FM
Reference
Layana et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

fluorescence donor

YFP tag

me31B 

Me31B

fluorescence donor

YFP tag

me31B 

Me31B

fluorescence donor

YFP tag

eIF4E3 

eIF4E-3

fluorescence acceptor

CFP tag

eIF4E3 

eIF4E-3

fluorescence acceptor

CFP tag

eIF4E3 

eIF4E-3

fluorescence acceptor

CFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; fluorescence donor produced from transfected construct; fluorescence acceptor produced from transfected construct.

(Layana et al., 2023)
FBrf0255814-7.coIP.WB
Reference
Layana et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eIF4E3 

eIF4E-3

bait
me31B 

me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Layana et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)