Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct in HEK293T cells.

synr can pull down Buffy and Debcl simultaneously.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct in HEK293T cells.

synr was cloned into a vector that includes tRNA synthetase and tRNA pair to incorporate pBPA into aa 124Y (of the BH3 domain), or 141F (near the BH3 domain) to stabilize the interaction. It was then incubated with transfected HA-tagged Buffy and uv-crosslinked.