A polyQ(80)pathogenic Hsap\ATXN3 was used. The UBS1 mutation increased interaction, suggesting it enhances Hsap\ATXN3 toxicity by compromising endogenous DnaJ-1 expression via a tighter interaction.

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

Source was adults of transgenic fly line; bait and prey produced from tagged transgenic constructs.

Hsap\ATXN3 contains a Q80 repeat.