FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kkv, SERCA
FlyBase ID
FBig0000122053
Interaction Network
Interactions Browser links
kkv network
SERCA network
Reported Interactions
FBrf0253598-1.TAP.MS
Reference
Zhu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kkv 

Kkv

bait

Strep, FLAG tag

SERCA 

SERCA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupa of wild type fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Zhu et al., 2022)
FBrf0253598-2.Y2H
Reference
Zhu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kkv 

Kkv

bait

fused to N-terminal ubiquitin fragment and LexA-VP16

kkv 
kkv 
kkv 
kkv 
SERCA 

SERCA

prey

fused to C-terminal ubiquitin fragment

SERCA 
SERCA 
SERCA 
SERCA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kkv 
transmembrane helix 2
sufficient binding region
aa 121-179

coordinates relative to kkv-PA

kkv 
transmembrane helix 6
sufficient binding region
aa 369-416

coordinates relative to kkv-PA

kkv 
transmembrane helix 7
sufficient binding region
aa 417-471

coordinates relative to kkv-PA

kkv 
transmembrane helix 8
sufficient binding region
aa 472-539

coordinates relative to kkv-PA

SERCA 
transmembrane helix 1
sufficient binding region
aa 48-81
SERCA 
transmembrane helix 3 and 4
sufficient binding region
aa 247-330
SERCA 
transmembrane helix 5
sufficient binding region
aa 743-783
SERCA 
transmembrane helix 7
sufficient binding region
aa 824-859
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhu et al., 2022)

Two-hybrid system: yeast split-ubiquitin. SERCA truncations interacted with kkv N terminal region aa 1-539.

(Zhu et al., 2022)
FBrf0253598-3.BiFC.FM
Reference
Zhu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kkv 

Kkv

bait

YFP tag, N-terminal fragment

SERCA 

SERCA

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live Sf9 cells; proteins produced from transfected constructs.

(Zhu et al., 2022)
FBrf0253598-4.PD.WB
Reference
Zhu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kkv 

Kkv

bait

Strep tag

SERCA 

SERCA

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhu et al., 2022)
FBrf0253598-5.coIP.WB
Reference
Zhu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kkv 

Kkv

bait

Strep tag

SERCA 

SERCA

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhu et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)