FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hts, mud
FlyBase ID
FBig0000122350
Interaction Network
Interactions Browser links
hts network
mud network
Reported Interactions
FBrf0257436-1.PD.MW
Reference
Parra et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hts 

Hts

bait

MBP tag

hts 
mud 

Mud

prey

His tag

mud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mud 
CC and PBD domains
sufficient binding region
aa 1760-1955

coordinates relative to mud-PF

hts 
N terminal head domain
necessary binding region
aa 1-401

coordinates relative to hts-PI

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Parra et al., 2023)
FBrf0263884-1.PD.MW
Reference
Seheon et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hts 

hts

bait

MBP tag

hts 
hts 
hts 
hts 
mud 

mud

prey

His tag

mud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mud 
Coiled coil domain
sufficient binding region
aa 1760-1890
hts 
Aldolase CTloop domain
mutation decreasing interaction
aa 331-352

deletion within the context of aa 110-401

hts 
Aldolase CThelix domain
mutation increasing interaction
aa 311-401

deletion within the context of aa 110-401

hts 
Aldolase YLE motif
mutation disrupting interaction
aa 159, 162 and 165

Y159A, L162A, E165A; deletion within the context of aa 110-401 or aa 1-310.

hts 
Aldolase domain residue 139
mutation decreasing interaction
aa 139

L139A; deletion within the context of aa 1-310.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Seheon et al., 2025)

Gels not shown for hts Aldolase YLE motif and Aldolase domain residue 139 mutations.

(Seheon et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (2)